N21-MAX Media Supplement (50X) Summary

For the reliable maturation and superior long-term culture of primary neurons.

Key Benefits

• Provides reliable maturation and superior function of neurons in culture
• Can be used to improve stem cell and 3D culture conditions
• Rigorous quality control testing ensures consistency across experiments
• Is nutrient-rich and serum-free
• Eliminates the need for an astrocyte feeder layer in neuronal cultures

Why Culture Neurons in Serum-Free N21-MAX Media Supplement?

The N21-MAX Media Supplement improves upon the traditional B27¹ and NS21² neuronal supplements, offering a serum-free and fully-defined formulation that is optimized for the reliable maturation, consistent health, and superior function of neurons in culture. There is an increase in the use of B27-like supplements to optimize stem cell and 3D cell culture media. N21-MAX improves stem cell differentiation and increases the viability and health of differentiated cell types during long-term cell culture conditions.

N21-MAX was designed to eliminate uncontrolled variables, such as those found in both serum-containing media and in some commercial serum-free neuronal supplements. Use of undefined media supplements can confound neuronal growth and maturation. N21-MAX contains a cocktail of factors optimized for neuronal survival, neurite extension, and synaptic maturation. Each lot of N21-MAX is tested on primary hippocampal neurons to ensure consistent performance for the user.

The N21-MAX Media Supplement:

• Is fully defined and quality tested to reduce unwanted experimental variation.
• Contains factors qualified to support reproducible and long-term neuron culture.
• Modified from the published B27 formulation¹.
• Does not require an astrocyte feeder layer.
• Has been tested to support the viability and growth of E18 rat hippocampal neurons.

1. Brewer et al., (1993) J. Neurosci. Res. 32:567
2. Chen et al., (2008) J. Neurosci. Methods 171:239

N21-MAX is optimized for the maturation and long-term culturing of neuronal cells. The supplement is supplied as a 50X concentrated solution and contains the following 21 components:

• Albumin (bovine)
• L-Carnitine
• Catalase
• Corticosterone
• Ethanolamine
• Glutathione
• Galactose
• Holo-Transferrin
• Insulin
• Linoleic Acid
• Linolenic Acid

• Lipoic Acid
• Progesterone
• Putrescine
• Retinyl acetate
• Retinol
• Selenite
• Superoxide dismutase
• Triiodo-L-thyronine
• D,L-alpha-Tocopherol
• D,L-alpha-Tocopherol acetate

Supplied in a volume sufficient to supplement 500 mL of media at the recommended concentration. N21-MAX is tested for use in Neurobasal Media.

Precautions

This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious agents, these reagents should be handled as if capable of transmitting infection.

Product Datasheets

Preparation and Storage

Background: Neural Stem Cells

Neural stem cells provide an excellent model for research focused on neural development and neurological disorders. R&D Systems offers ready-to-use primary cortical stem cells isolated from E14.5 Sprague-Dawley rats. In addition, primary mouse cortical stem cells isolated from E14.5 CD-1 mice are available. Every lot of R&D Systems Cortical Stem Cells is validated for a high level of Nestin expression and the capacity for multi-lineage differentiation into astrocytes, neurons, and oligodendrocytes. Our cortical stem cells are tested to ensure highest quality and lot to lot consistency. Both rat and mouse Cortical Stem Cells can be optimally expanded as monolayers or neurospheres.

To complement the use of primary neural stem cells, we offer a range of supportive products, including culture media which is specifically optimized for use with neural stem cells. We offer kits to promote the in vitro proliferation of neural precursors, and kits to differentiate neural stem cells into dopaminergic neurons or oligodendrocytes. In addition, kits are available which contain panels of antibodies designed to monitor the differentiation and identification of neural precursors, astrocytes, neurons, and oligodendrocytes.

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, completed N21-MAX-supplemented medium is prepared using the following procedure:

• Dilute the media supplement in basal media
• Store completed media
• Use within 2 weeks

Reagents provided in the N21-MAX Media Supplement (Catalog # AR008):

• Albumin (bovine)
• L-Carnitine
• Catalase
• Corticosterone
• Ethanolamine
• Glutathione
• Galactose
• Holo-Transferrin
• Insulin
• Linoleic Acid
• Linolenic Acid
• Lipoic Acid
• Progesterone
• Putrescine
• Retinyl acetate
• Retinol
• Selenite
• Superoxide dismutase
• Triiodo-L-thyronine
• D,L-alpha-Tocopherol
• D,L-alpha-Tocopherol acetate

Reagents

• Basal media (e.g., Neurobasal Media from Invitrogen^®, Catalog # 21103-029 or equivalent)
• L-Glutamine

Materials

• Serological pipettes
• Pipettes and pipette tips

Equipment

• 2 °C to 8 °C refrigerator

Dilute 50-fold with a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029) and supplement with 0.5 mM L-glutamine before use. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.

Invitrogen is a registered trademark of Invitrogen Corp.

Citations for N21-MAX Media Supplement (50X)

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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1. Stemness-Attenuating miR-503-3p as a Paracrine Factor to Regulate Growth of Cancer Stem Cells
   Authors: M Seo, SM Kim, EY Woo, KC Han, EJ Park, S Ko, EW Choi, M Jang
   Stem Cells Int, 2018;2018(0):4851949. 2018
   
2. Axonal G3BP1 stress granule protein limits axonal mRNA translation and nerve regeneration
   Authors: PK Sahoo, SJ Lee, PB Jaiswal, S Alber, AN Kar, S Miller-Ran, EE Taylor, T Smith, B Singh, TS Ho, A Urisman, S Chand, EA Pena, AL Burlingame, CJ Woolf, M Fainzilber, AW English, JL Twiss
   Nat Commun, 2018;9(1):3358. 2018
   
3. Development of a central nervous system axonal myelination assay for high throughput screening.
   Authors: Lariosa-Willingham K, Rosler E, Tung J, Dugas J, Collins T, Leonoudakis D
   BMC Neurosci, 2016;17(0):16. 2016
   

FAQs

1. What advantages does the N21-MAX Media Supplement (Catalog # AR008) have over other neuronal stem cell supplements?

   • We have performed comparison testing in-house and have found that in long term cultures (21 days), we observe better synapse marker expression.
     Additionally, one major formulation difference is that our N21 contains holo-transferrin while other supplements typically contain apo-transferrin. It is reported in the literature that holo-transferrin is preferred for neuronal cell culture. We have also tested several BSA sources to determine which is optimal for neuronal cultures.

Reviews for N21-MAX Media Supplement (50X)

Average Rating: 4.4 (Based on 5 Reviews)

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The area, intensity, and density of PSD-95 signal are comparable to the culture grown in B27.

Adult human stem cells showed fast growth and took less time to differentiate into neurons. Data is communicated so cannot upload image.

I have previously used Gibco B27®, and Gem21 NeuroPlex™ for culturing of neural cells and neuronal lines. I use typical for co- culture of neurons with astrocytes and microglia . I tried your sample N21 max from R&D and it worked very well with my neuronal cells co cultures. All of these Gibco B27®, and Gem21 NeuroPlex™ and N21 max are Supplied as a 50X concentrate and are list priced at $79 for 10 ml. I would use them all interchangeably as they all worked equally well in my co culture system.

For stem cell culture media supplementation.

We collected the majority of metadata history records for Ns21.me. Ns 21 has an elaborated description which rather positively influences the efficiency of search engines index and hence improves positions of the domain. The description and keywords of Ns21 were last changed more than 2 months ago.